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9780801888083 Academic Inspection Copy

Protein Crystallography

A Concise Guide
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The proteome remains a mysterious realm. Researchers have determined thestructures of only a small fraction of the proteins encoded by the human genome.Crystallography continues to be the primary method used to determine the structuresof the remaining unknown proteins. This imaging technique uses the diffraction ofX-rays to determine a protein's three-dimensional molecular structure.Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundantillustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights.The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

Preface1. Introduction1.1. What Is X-ray Crystallography?1.2. A Quick Look at Protein Crystals1.3. Noncrystalline Specimens1.4. SummaryFurther Reading2. A Physical Understanding of Diffraction2.1. What Is Diffraction?2.2. Diffraction from One-Dimensional Crystals2.3. Reconstructing Images from Diffraction Patterns2.4. SummaryFurther Reading3. Diffraction from Three-Dimensional Crystals3.1. The Electron Density Function in Three Dimensions3.2. Calculating the Diffraction Pattern from a Known Structure3.3. SummaryFurther Reading4. Phase Determination by Isomorphous Replacement4.1. Measuring the Phases4.2. MAD Phasing4.3. Fitting Models to Experimental Electron Density Maps4.4. SummaryFurther Reading5. The Patterson Function5.1. Definition of the Patterson Function5.2. Using the Patterson Function to Locate Atoms5.3. SummaryFurther Reading6. Phasing with Partially Known Structures6.1. Difference Fourier Maps6.2. Molecular Replacement6.3. SummaryFurther Reading7. Crystallographic Refinement7.1. Refinement Improves the Model7.2. Least-Squares Refinement7.3. SummaryFurther ReadingGlossaryIndex

""One of the main assets of the book is its many illustrations. Without exception the figures are straightforward to interpret, and the visual support enables the newcomers to the field to follow the presented material easily.""

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